Cloning and Characterization of a Tissue Specific Promoter GluB-1 from Nipponbare by Transformation in Rice

Authors

  • Sarah Sarker, Rumana S. Tammi, Sudip Biswas, Shabnam Zaman and Zeba I. Seraj* Plant Biotechnology Lab, Department of Biochemistry and Molecular Biology, University of Dhaka, Bangladesh

Keywords:

glutelin, Nipponbare cultivar, Agrobacterium-mediated transformation

Abstract

Tools of plant biotechnology can be applied as a routine procedure for improving rice grain quality or production of desired recombinant proteins in the rice seeds. For this purpose, endosperm specific promoter is a useful choice as desired gene expression would take place only in rice seed and not in root or shoot. Also, rice seed has become an attractive bioreactor for the production of recombinant proteins compared to other cereals. Glutelins are the most abundant storage proteins in rice grain which constitute up to 80% of the total protein content. The promoter region of GluB-1, one of the glutelin genes in rice, has been used as a model to study the regulation of seed-storage protein accumulation. In this study the upstream region (2.4 kb) of the GluB-1 gene was amplified from the genomic DNA of the Nipponbare cultivar of Oryza sativa (japonica group) and then cloned successively into an entry and promoter-characterization binary destination vector having the reporter gene B-glucuronidase (GUS) by applying Gateway Technology. Three plants generated by Agrobacterium mediated tissue culture were confirmed by PCR with GluB-1 specific primer. In the transformed plants, histochemical GUS assay showed no expression in the root and shoot but was prominent in the endosperm of the T1 seeds. Therefore this 2.4 kb promoter from Nipponbare rice can be successfully used to improve rice grain quality or express stable recombinant proteins in rice seeds for therapeutic purposes.

 

Citation
This paper has been cited by the following papers:

1. Cloning, characterization and analysis of the Arabidopsis RD29A promoter for its inducible expression in rice under salinity and drought stress
2. Heterologus Protein Expression in Different Host Systems
3. Cloning and characterisation of an endosperm specific glutelin B1 promoter from Sri Lankan rice (Oryza sativa L. ssp. indica) variety Bg 250
4. Agrobacterium Mediated Transformation of OsTZF8 Gene in Oryza sativa for Drought Stress Tolerance.

How to Cite

Sarah Sarker, Rumana S. Tammi, Sudip Biswas, Shabnam Zaman and Zeba I. Seraj* (1) “Cloning and Characterization of a Tissue Specific Promoter GluB-1 from Nipponbare by Transformation in Rice”, Bioresearch Communications-(BRC). Dhaka, Bangladesh, 1(2), pp. 69-75. Available at: http://bioresearchcommunications.com/index.php/brc/article/view/48 (Accessed: 29November2020).

Issue

Section

Original Article