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Optimization of different carbon, nitrogen and amino acid substrates and their effects for bioethanol production by Escherichia coli DH5α


Yasmin Akter1*, Lolo Wal Marzan1, Md Amzad Hossain2,Yeasmeen Ali1 Md. Arifuzzaman3

1Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chittagong- 4331, Bangladesh.2Department of Genetic Engineering and Biotechnology, Jagannath University, Dhaka 1100, Bangladesh.3Department of Biochemistry and Molecular Biology, University of Science and Technology, Chittagong, Bangladesh.

ABSTRACT: This study aims to discover the effect of different carbon, nitrogen and amino acid substrates for better ethanol production by Escherichia coli DH5α, where pH 7.0± 0.5 and 37°C is suitable for their cell growth at 12 hours incubation period during 150 rpm. The effects of different carbon sources were assessed during the exponential phase of E. coli DH5α, where complete consumption of different carbohydrates and amino acids were observed. Moreover, glucose with trehalose supplement induced cell growth and producing 0.15± 0.01 g/L ethanol at 28 hours shake flask culture measured by redox titration method. Ammonium sulphate and serin increased cell growth compared to other, while trehalose supplement with glucose (in M9 media) can increase cell growth and metabolite (ethanol) production. Therefore, this optimized and suitable condition for E. coli DH5α might be a base study to develop industrial energy production to fulfill partial demand in future.

KEYWORDS: Escherichia coli DH5α, Optimization, Culture conditions, Trehalose, Bioethanol

CORRESPONDENCE: Yasmin Akter

 

 
 
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